Journal
CELLULAR AND MOLECULAR BIOLOGY
Volume 66, Issue 5, Pages 142-147Publisher
C M B ASSOC
DOI: 10.14715/cmb/2020.66.5.25
Keywords
Resveratrol; Skin squamous cell carcinoma; miR-126; Cell proliferation; Migration and invasion; Wnt / beta-catenin signaling pathway
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To explore the effect and possible mechanism of resveratrol on the proliferation, migration and invasion of skin squamous cell carcinoma cells (HSC-5). Tetramethyl azozo blue (MTT) method, Transwell experiment and real-time fluorescence quantitative PCR (RT-qPCR) were used to detect the effects of resveratrol intervention on the proliferation, migration and invasion of HSC-5 cells and the expression of miR-126. The miR-126 mimics and inhibitors were transfected into HSC-5 cells, and the effects of up-regulation or down-regulation of HSC-5 expression on the proliferation, migration and invasion of HSC-5 cells were detected. The miR-126 inhibitor was transfected into HSC-5 cells, and the effects of resveratrol intervention on HSC-5 cell proliferation, migration and invasion, and beta-catenin protein expression were detected. After resveratrol intervention, the growth rate, migration and invasion of HSC-5 cells were significantly reduced, miR-126 expression was significantly increased, and beta-catenin protein expression was significantly reduced (p<0.05). After up-regulating the expression of miR-126, the growth rate, migration and invasion of HSC-5 cells were significantly reduced (p<0.05). After down-regulating the expression of miR-126, the growth rate, migration and invasion of HSC-5 cells were significantly increased (p<0.05). Down-regulation of miR-126 expression could reverse the effects of resveratrol intervention on HSC-5 cell proliferation, migration and invasion, and beta-catenin protein expression (p<0.05). Resveratrol could inhibit the proliferation, migration and invasion of skin squamous cell carcinoma cells, which may be related to the up-regulation of miR-126 to inhibit the Wnt / beta-catenin signaling pathway.
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