Journal
INTERNATIONAL IMMUNOPHARMACOLOGY
Volume 42, Issue -, Pages 32-38Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.intimp.2016.11.010
Keywords
Paeonol; Physicochemical properties; 2,4,6-trinitrobenzene sulfonic acid; Ulcerative colitis
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Funding
- National Natural Science Foundation of China [81303233]
- Shanghai Committee of Science and Technology [13401900300]
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Paeonol, an active component from Paeonia suffruticosa Andr., has a variety of biological activities, such as vascular endothelial cell protection, anti-oxidation, and anti-inflammation. The aim of this study was to investigate the basic physicochemical properties of paeonol, including solubility, oil-water partition coefficient, and permeability. Then evaluated the anti-inflammatory effects of paeonol were evaluated on 2,4,6-trinitrobenzenesulfonic acid-induced ulcerative colitis in rats. The rats were divided randomly into 6 groups, namely, normal, model, paeonol-treated (100, 200, and 400 mg/kg), and positive. Each group had 10 rats. Inhibition effects were evaluated by the disease activity index (DAI), colon weight/length ratio, as well as macroscopical and histological evaluations. Serum interleuldn (IL)-17, IL-6 and transforming growth factor beta 1 (TGF-beta 1) levels were determined by enzyme-linked immunosorbent assay. The solubility and oil-water partition coefficient of paeonol in different phosphate buffer solutions were 284.06-598.23 and 461.97-981.17 mu g/mL, respectively. The effective passive permeability value Pe was 23.49 x 10(-6) cm/s. In terms of anti-inflammatory results, compared with the model group, treatment with 200 and 400 mg/kg doses of paeonol had significantly decreased DAI, colon weight/length ratio, and macroscopic and histopathological scores. Furthermore, the serum levels of IL-17 and IL-6 were significantly reduced, whereas the TGF-beta 1 level was increased in the two paeonol-treated groups (medium- and high dose group). Therefore, paeonol had poor water solubility, but oral absorption was good. In addition, paeonol had therapeutic effects on ulcerative colitis, and the therapeutic efficacy was dose dependent. The results presented in this study provide evidence for the development of a novel therapeutic agent in the treatment of UC. However, whether this agent could have therapeutic benefit or adverse effects in human IBD remains to be fully explored. (C) 2016 Published by Elsevier B.V.
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