Journal
INTERNATIONAL IMMUNOPHARMACOLOGY
Volume 50, Issue -, Pages 14-21Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.intimp.2017.06.004
Keywords
Hyperoside; Microglia; Neuroinflammation; Lipopolysaccharide; Parkinson's disease
Categories
Funding
- Zhejiang Provincial Natural Science Foundation [LY16H310012]
- Zhejiang Provincial Bureau of Chinese Medicine [2016ZB078]
- Wenzhou Science and Technology Bureau [Y20150005, Y20160064]
- Wenzhou Medical University [89215020]
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Hyperoside (quercetin-3-O-beta-D-galactoside) is an active compound isolated from herbs. Neuroinflammation is a key mechanism involved in neurodegenerative disorders including Parkinson's disease. In this study, we aimed to investigate the potentiality of hyperoside in inhibiting microglia-mediated neuroinflammation. BV2 microglial cells were pretreated with hyperoside and stimulated with lipopolysaccharide (LPS). The results showed that hyperoside significantly inhibited LPS-induced production of nitric oxide and pro-inflammatory cytokines including IL-1 beta and TNF-alpha, as well as the expression of inducible nitric oxide synthase. Similar results were observed in primary microglial cells isolated from neonatal mice. Analyses in MAPK and NF kappa B signaling combined with specific inhibitors suggested that hyperoside attenuated the LPS-induced inflammatory responses via p38 and NF kappa 3 pathways. Furthermore, hyperoside suppressed reactive microglia-mediated neurotoxicity as evidenced by conditioned media culture, but had no direct impact on MPP+-induced toxicity in SH-SY5Y neuroblastoma cells. Collectively, our data suggest that hyperoside may serve as a protective agent by alleviating microglia activation in disorders such as Parkinson's disease.
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