Journal
INTERNATIONAL BIODETERIORATION & BIODEGRADATION
Volume 119, Issue -, Pages 649-660Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.ibiod.2016.09.019
Keywords
TeCA biodegradation; Aerobic cometabolism; Alkane monooxygenase; Bioreactor configuration; Full-scale process simulation
Funding
- Vasco e GC Rossi - Microbial Biofilm grant [A.10.N4.RICER.797]
- University of Bologna
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Microbial degradation of 1,1,2,2-tetrachloroethane has been rarely analysed under aerobic conditions. In this work, the catabolic potential of a TeCA-degrading aerobic propanotroph consortium (C-2) and the optimal bioreactor configuration for an on-site TeCA-bioremediation strategy with C-2 were defined. More specifically, the diversity of alkane-oxidizing bacteria in C-2 was assessed by means of clone libraries of genes coding for alkane monooxygenases (MOs) of different families (AIkB-like alkane hydroxylase, soluble di-iron MO and cytochromes P450). A large number of alkane MO sequences retrieved in this study showed the highest similarity with reference sequences belonging to Rhodococcus genus, suggesting a key role of this genus in TeCA/propane co-metabolism, while the remaining alkane MO sequences were mainly attributed to other Actinobacteria, to Bradhyrizobiaceae, and Cupriavidus genus. Further, the feasibility of an on-site TeCA bioremediation strategy with C-2 was evaluated by simulating a continuous-flow aerobic co-metabolic process with different bioreactor configurations. Our results show that the configuration with a suspended-cell continuous stirred-tank reactor (CSTR) followed by a suspended-cell plug-flow reactor (PFR) was the one giving the best performance with consortium C-2. (C) 2016 Elsevier Ltd. All rights reserved.
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