Journal
MATERIALS SCIENCE AND ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
Volume 116, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.msec.2020.111255
Keywords
Apoptosis, cellular uptake; Magnetic hyperthermia; Methotrexate; Nanostructured lipid carrier; Superparamagnetic iron oxide nanoparticles
Categories
Funding
- European Union
- National Fund (FCT/MEC) [PT2020 UID/QUI/50006/2020]
- National Fund (Ministerio da Educacao e Ciencia) [PT2020 UID/QUI/50006/2020]
- FCT/MEC - national funds [CEECIND/01620/2017]
- NORTE 2020 (2014-2020 North Portugal Regional Operational Program)
- ERDF (European Regional Development Fund) [NORTE-01-0145-FEDER-000019]
- FCT
- ERDF through NORTE2020
- COST Action RADIOMAG [TD1402]
- National Fund (Fundacao para a Ciencia e a Tecnologia) [PT2020 UID/QUI/50006/2020]
- project (Nanother) [UTAP-EXPL/NTec/0038/2017]
- project (MagtargetON) [NORTE-01-0145-FEDER-031142]
Ask authors/readers for more resources
Methotrexate (MTX), an anti-neoplastic agent used for breast cancer treatment, has restricted clinical applications due to poor water solubility, non-specific targeting and adverse side effects. To overcome these limitations, MTX was co-encapsulated with an active-targeting platform known as superparamagnetic iron oxide nanoparticles (SPIONs) in a lipid-based homing system, nanostructured lipid carrier (NLC). This multi-modal therapeutic regime was successfully formulated with good colloidal stability, bio- and hemo-compatibility. MTX-SPIONs co-loaded NLC was time-dependent cytotoxic towards MDA-MB-231 breast cancer cell line with IC50 values of 137 mu g/mL and 12 mu g/mL at 48 and 72 h, respectively. The MTX-SPIONs co-loaded NLC was internalized in the MDA-MB-231 cells via caveolae-mediated endocytosis in a time-dependent manner, and the superparamagnetic properties were sufficient to induce, under a magnetic field, a localized temperature increase at cellular level resulting in apoptotic cell death. In conclusion, MTX-SPIONs co-loaded NLC is a potential magnetic guiding multi-modal therapeutic system for the treatment of breast cancer.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available