Journal
CURRENT DRUG TARGETS
Volume 16, Issue 13, Pages 1488-1494Publisher
BENTHAM SCIENCE PUBL LTD
DOI: 10.2174/1389450116666150120105133
Keywords
(1 -> 3)-alpha-D-glucan; Aspergillus; carboxymethylation; cytotoxicity; human cell cultures
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Funding
- BW/BS/BiB/UMCS Research Programs
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Alkali-soluble polysaccharides (ASPs) were isolated from the cell wall of four Aspergillus species (A. fumigatus, A. nidulans, A. niger, and A. wentii). The chemical and spectroscopic investigations (immunofluorescent labelling, composition analysis, methylation analysis, FTIR, and H-1 NMR) indicated that the ASPs were polymers composed almost exclusively of (1 -> 3)-linked alpha-D-glucose. After carboxymethylation (CM), the activity of (1 -> 3)-alpha-D-glucans on three human cell lines (HSF, HeLa, and Jurkat) was assessed. Anti-proliferative, cytotoxic, and free radical scavenging action of CM-alpha-D-glucans was analysed. All the tested CM-alpha-D-glucans decreased cellular metabolism. However, incubation with CM-alpha-D-glucan from A. wentii and A. niger increased (by ca. 50%) the viability of HSF cells. Moreover, an over 5-fold increase in the viability was found for Jurkat cells incubated with CM-alpha-D-glucans from A. fumigatus and A. nidulans. The CM-(1 -> 3)-alpha-D-glucans from the tested Aspergillus species expressed no free radical scavenging action. Fluorescent staining revealed that CM-alpha-D-glucans exerted slight toxic effects on cell viability and no action on F-actin filaments of cellular cytoskeleton organization.
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