Journal
STAR PROTOCOLS
Volume 1, Issue 2, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.xpro.2020.100073
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Funding
- Fund for Scientific Spearheads of Ghent University Hospital
- Concerted Research Actions from Ghent University
- Stichting tegen Kanker
- Fund for Scientific Research-Flanders
- Kom op tegen Kanker (Stand up to Cancer), the Flemisch cancer society
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The multidimensional cargo of extracellular vesicles (EV) released in urine is a reflection of the pathophysiological processes occurring within their cells and tis-sues of origin in the urogenital system. Here, we describe a step-by-step protocol for density-based separation of urinary EV with high specificity and repeatability. The implementation of integrative omics allows the study of the molecular complexity of highly purified urinary EV, supporting the identification of EV-spe-cific functions and biomarkers. For complete details on the use and execution of this protocol, please refer to Dhondt et al. (2020).
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