Journal
INFLAMMATION
Volume 40, Issue 5, Pages 1606-1621Publisher
SPRINGER/PLENUM PUBLISHERS
DOI: 10.1007/s10753-017-0601-x
Keywords
TSP-1; P. gingivalis LPS; IL-6; IL-1 beta; TNF-alpha; NF-kappa B signaling; PDTC
Categories
Funding
- National Natural Science Foundation of China [81600845]
- Provincial Natural Science Research Project of Colleges
- Universities of Anhui Province [KJ2016A348]
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Thrombospondin-1 (TSP-1) is upregulated in several inflammatory diseases. Recent data have shown that macrophages from TSP-1-deficient mice have a reduced inflammatory phenotype, suggesting that TSP-1 plays a part in macrophage activation. DNA microarray approach revealed that Porphyromonas gingivalis lipopolysaccharide (P. gingivalis LPS) may induce the enhanced TSP-1 expression in human monocytes, suggesting a role of TSP-1-mediated pathogenesis in periodontitis. Until recently, the function of TSP-1 has been a matter of debate. In this study, we explored the role of TSP-1 in inflammatory cytokine secretions and its putative mechanism in pathogenesis of periodontitis. We demonstrated that TSP-1 expression was significantly upregulated in gingival tissues with periodontitis and in P. gingivalis LPS-stimulated THP-1 cells. Deficiency of TSP-1 by transfecting siRNAs decreased IL-6, IL-1 beta, and TNF-alpha secretions in THP-1 cells, whereas overexpression of TSP-1 resulted in an upregulation of IL-6, IL-1 beta, and TNF-alpha productions. Additional experiments showed that Pyrrolidine dithiocarbamate (PDTC) inhibited IL-6, IL-1 beta, and TNF-alpha expression induced by overexpression of TSP-1, accompanying with downregulation of phosphorylated p65 and I kappa B alpha protein levels in response to P. gingivalis LPS. These results indicated that TSP-1 played a significant role in P. gingivalis LPS-initiated inflammatory cytokines (IL-6, IL-1 beta, and TNF-alpha) secretions of THP-1 cells, and the NF-kappa B signaling is involved in its induction of expression. Thus, TSP-1 effectively elevated P. gingivalis LPS-induced inflammation mediated by the NF-kappa B pathway and may be critical for pathology of periodontitis.
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