Analysis of Anelloviridae sequences characterized from serial human and animal biological samples

Rolling-circle amplification-sequence-independent single primer amplifications (RCA-SISPA) and/or RCA-PCRbased approaches were applied to serial human plasma and animal (domestic cat) saliva samples. Complete SENV-H-related and PRA4 Anelloviridae genomes were characterized and analysed over time (similar to tative coding regions were compared. Comparable values, i.e. similar to 2 x 10(-4) subs/site/year, were obtained for estimated rates of non-synonymous substitutions. A hot-spot of mutations located on the SENV-H-related ORF1 was identified. These results are first data concerning Anelloviridae evolution in a human and an animal host based on the analysis of complete sequences. (C) 2017 Elsevier B.V. All rights reserved.