Chemical analysis of the characteristics of Tunisian Juglans regia L. fractions: Antibacterial potential, gas chromatography-mass spectroscopy and a full investigation of their dyeing properties

The current report deals with the assessment of the characteristics of Tunisian Juglans regia L. (Walnut) fractions. First, prepared stem and leaves methanolic extracts were chemically characterized. The Total Phenolic Contents (TPC), Total Flavanoid Contents (TFC) and the concentration required to scavenge 50% of DPPH* (IC50) were determined. The ability of walnut fractions to quench reactive species was measured through 1,1-diphenyl-1-picrylhydrazyl (DPPH*) radical scavenging activity assay. The highest values of TPC and TFC were achieved using leaves fraction whereas the maximum value of IC50 was reached using stem part. The main constituents of this latter were analyzed using Gas Chromatography-Mass Spectroscopy (GC MS) and its antibacterial activities against pathogenic strains of Aspergillus Niger, Aeoromonas hydrophila, Pseudomonas Fluorescens, Salmonella arizonae 2, Listeria monocytogenes, Staphylococcus aureus, Salmonella arizonael, Orchi Epididymite and Escherichia Coll were reported. Results reveal that Aspergillus Niger and Salmonella arizonae I are the most sensitive microorganisms exhibiting the largest inhibition zones (18 < d < 20 mm). The dyeing properties of stem, leaves and their mixture with various arrangements were performed on wool, cotton and polyamide. The effect of the mode of dyeing, pH value, extract concentration, temperature and agitation speed were studied and the dyeing performances of samples were determined. For example, compared to the dyeing properties using leaves extract at optimum conditions, the improvement of color in the leaves-stem mixture is about 1.7 times. The resulting data from rub, light, wash and perspiration fastness assays exhibited fair to excellent grades.