PARTIAL PURIFICATION AND KINETIC CHARACTERIZATION OF RENAL CATALASE FROM CARNIVOROUS FISH, Channastriata EXPOSED TO PESTICIDES MIXTURE,DELTAMETHRIN plus ENDOSULFAN

The current work was accomplished to purify the renal catalase (CAT) from carnivorous fish, Channa striata exposed to sublethal dose (1/3rdof LC50) of deltamethrin (DM)+endosulfan(END) mixture. Fish was kept under control and experimental conditions for 14-days. Water quality characteristics like temperature, total hardness and pH were kept stabilized as 30 degrees C, 250 mgL(-1) and 7.5, respectively. The biochemical technique Ammonium sulphate precipitation was used for partial purification of CAT. In Kinetic characterization optimum pH and temperature was determined for CAT. Spectrophotometer was used to analyze activity of enzyme. Results of isolation showed that activity of CAT was lowest in crude extract of DM+END exposed C. striata renal tissue as compared to control. Similarly, specific activity of CAT was also lowest in crude extract of exposed C. striata renal tissue as compared to control. As a result of partial purification of renal CAT, lowest specific activity was observed in exposed C. striata (80.94 +/- 0.00 Umg(-1)) than control (88.97 +/- 1.41 Umg(-1)).The fold purification of renal CAT from exposed and control fish was calculated as 1.24 +/- 0.00 and 1.28 +/- 0.01, respectively. The percentage recovery was about 58.07 +/- 1.41 and 63.23 +/- 1.41 % for exposed and control C. striata, respectively. Kinetic characterization of CAT showed that the maximum activity was observed at pH 6.5 and temperature 30 degrees C.