Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 323, Issue -, Pages -Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2020.128655
Keywords
Photoelectrochemical biosensor; Toehold-mediated strand displacement reaction; Bi(2)S(3)nanoflower; Let-7a
Funding
- National Natural Science Foundation of China [21775055, 21874055]
- Shandong Province Higher Educational Youth Innovation Science and Technology Program [2019KJC016]
- Taishan Scholars programand Case-by-Case Project for Top Outstanding Talents of Jinan
- Project of 20 items of University of Jinan [2018GXRC001]
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Herein, a photoelectrochemical (PEC) biosensor we proposed was used to detect let-7a via studying the photocurrent response change resulted from the change of the toehold-mediated strand displacement reaction (TSDR) between the AuNPs-H-2 (a conjugate of gold nanoparticles and DNA strand H2) and let-7a. Without the participation of let-7a, after the alkaline phosphatase (ALP) were immobilized at Bi2S3 surface through modified on the AuNPs-H2, ALP converted the ascorbic acid 2-phosphate (AAP) to produce ascorbic acid (AA), AA could be catalytically oxidized to provide electrons, resulting in the enhancement of photocurrent signal on the Bi-2 S-3. In contrast, the conjugate of ALP@AuNPs-H2 (a conjugate of ALP and AuNPs-H2) were compelled away from Bi2S3 surface when the let-7a participated in the process and hybridized with HI The fuel DNA assisted the recycling of the let-7a via another TSDR, the recovered let-7a could participate in the next enzyme-free cycle. The PEC biosensor not only exploited TSDR signal amplification strategy, but also achieved good linearity in the range of 0.01 nM-1000 nM with a low detection limit of 6.7 pM. Due to the excellent performance, we believe it will offer more opportunities for bioanalysis and clinical biomedicine.
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