Journal
LAB ON A CHIP
Volume 20, Issue 19, Pages 3612-3624Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/d0lc00675k
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Funding
- National Research Foundation of Korea (NRF) - Korean government (MSIP) [U24-AI118656]
- [2017R1A6A3A11036525]
- [2020R1A2C2007017]
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A fully-automated and portable leukocyte separation platform was developed based on a new type of inertial microfluidic device, multi-dimensional double spiral (MDDS) device, as an alternative to centrifugation. By combining key innovations in inertial microfluidic device designs and check-valve-based recirculation processes, highly purified and concentrated WBCs (up to >99.99% RBC removal, similar to 80% WBC recovery, >85% WBC purity, and similar to 12-fold concentrated WBCs compared to the input sample) were achieved in less than 5 minutes, with high reliability and repeatability (coefficient of variation, CV < 5%). Using this, one can harvest up to 0.4 million of intact WBCs from 50 mu L of human peripheral blood (50 mu L), without any cell damage or phenotypic changes in a fully-automated operation. Alternatively, hand-powered operation is demonstrated with comparable separation efficiency and speed, which eliminates the need for electricity altogether for truly field-friendly sample preparation. The proposed platform is therefore highly deployable for various point-of-care applications, including bedside assessment of the host immune response and blood sample processing in resource-limited environments.
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