Journal
BIOSENSORS & BIOELECTRONICS
Volume 168, Issue -, Pages -Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2020.112559
Keywords
Lateral flow; Sensitivity enhancement; Wax barrier; Point-of-Care; Optical biosensor
Categories
Funding
- NACANCELL project [PCIN2016-066]
- CERCA Program/Generalitat de Catalunya
- Spanish MINECO [MAT 2017-87202-P]
- Severo Ochoa Centres of Excellence Program [SEV2201320295]
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The ease of use, low cost and quick operation of lateral flow assays (LFA) have made them some of the most common point of care biosensors in a variety of fields. However, their generally low sensitivity has limited their use for more challenging applications, where the detection of low analytic concentrations is required. Here we propose the use of soluble wax barriers to selectively and temporarily accumulate the target and label nanoparticles on top of the test line (TL). This extended internal incubation step promotes the formation of the immune-complex, generating a 51.7-fold sensitivity enhancement, considering the limit of quantification, and up to 96% signal enhancement compared to the conventional LFA for Human IgG (H-IgG) detection.
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