Photonic crystal surface mode imaging for multiplexed and high-throughput label-free biosensing

A photonic crystal surface mode imaging (PCSMi) technique is implemented for the simultaneous detection of antibody binding with specific antigens in arrays containing 96- and 384-spots. Like the surface plasmon resonance imaging (SPRi) technique, the presented approach is label-free and permits interrogating an analyte by hundreds of different ligands immobilized in small spots. The adsorption kinetics is recorded with a subpicogram resolution at every spot simultaneously. Possible implementations of this technique for multiplexed and high-throughput biosensing are discussed.