Journal
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL
Volume 53, Issue 10, Pages 888-895Publisher
SPRINGER
DOI: 10.1007/s11626-017-0214-6
Keywords
Cryogenic; Cryopreservative; Inventory system; Liquid nitrogen freezers; Low-temperature storage; Mechanical freezers; Safety; Temperature gradient; Thermal risk; Validation; Dangerous goods; Dry ice; Labeling; Liquid nitrogen; Packaging; Shipment
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Successfully recovering stored cryopreserved cells requires attention to two major factors, i.e., storage temperature and proper handling of the frozen samples. These key considerations are in turn impacted by how the inventory system is constructed, the frequency and process for retrieving specimens, as well as how the material is secured. In addition, shipping biological materials requires attention to the type of material transported, adherence to regulatory requirements, packaging materials and proper assembly, labeling, and engaging reputable carriers. This paper provides an overview discussion of the current best practices for storing, handling, and shipping of cryopreserved cells and provides references to more detailed information on specific topics.
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