Journal
ANALYST
Volume 145, Issue 19, Pages 6277-6282Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/d0an01224f
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Funding
- NSFC [81773894]
- Natural Science Foundation of Fujian Province, China [2018J01871, 2019Y4009, 2017Y0047]
- Natural Science Funds of Fujian Province for Distinguished Young Scholar [2019J06021]
- Open Project of Jiaxing University Key Laboratory of Rapid Detection Technology and Instruments for Environment and Food
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An enzyme-catalyzed fluorescence switch type sensor was constructed for the determination of alkaline phosphatase (ALP) activity by combining the fluorescence quenching effect of Ag(+)on ultrathin g-C(3)N(4)nanosheets (CNNSs) with the simple redox reaction of AA and Ag+. Briefly, Ag(+)exhibits a significant quenching effect on the fluorescence of CNNSs. Thus the fluorescence signal of the CNNS-Ag(+)system is extremely weak even in the presence ofl-ascorbic acid-2-phosphate (AAP) (off state). When ALP coexists in the system, the enzyme can specifically catalyze the hydrolysis of AAP to form ascorbic acid (AA), which reduces Ag(+)to Ag-0. In this case, the fluorescence signal of the system is recovered (on state). Based on this principle, a signal-enhanced CNNS fluorescence sensor was developed to determine the activity of alkaline phosphatase. The experimental results show that the detection range of alkaline phosphatase is 0.5-20 U L-1, and the detection limit is 0.05 U L-1(S/N = 3). Meanwhile, this method was used to assay ALP in serum samples.
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