Journal
IMMUNOLOGY LETTERS
Volume 189, Issue -, Pages 101-108Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.imlet.2017.04.010
Keywords
Dermatology; Flow cytometry; Circulating follicular t helper cells; Interleukin-21; SCORAD
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Funding
- DEFENSE-NET project [TAMOP 4.2.2.A-11/1/KONV-2012-0023]
- European Social Fund
- European Regional Development Fund
- [OTKA K-101470]
- [OTKA K-108421]
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Follicular helper T (T-FH) cells play crucial role in B-cell differentiation and antibody production. Although, atopic dermatitis (AD) is often associated with increased serum IgE levels, B-cell mediated responses have not been studied thoroughly. The aim of our study was to investigate the proportion of T-FH-like cells in the disease. Twelve children and 17 adults with AD as well as 14 healthy controls were enrolled in the study. The frequency of CD4(+)CXCRS(+)ICOS(+)PD-1(+) T-FH-like cells and their IL-21 cytokine production were determined by flow cytometry. Immunohistochemical analysis was performed on skin biopsy specimens from AD patients for the detection of T-FH markers. The percentages and absolute numbers of circulating T-FH-like cells were significantly increased in children with AD compared to adult patients and healthy controls. IL-21 cytokine production of T-FH-like cells was also elevated and showed a strong positive correlation with paediatric patients' SCORAD index. The expression of T-FH-specific markers showed only a non-specific scattered pattern in skin biopsy specimens. This is the first study to demonstrate that T-FH-like cells expanded in the peripheral blood of children with AD compared to adults. These results reinforce the importance of further investigations on T-FH-like cells in different phenotypes and endotypes of AD.
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