Journal
IMMUNOLOGY
Volume 152, Issue 3, Pages 414-424Publisher
WILEY
DOI: 10.1111/imm.12776
Keywords
1, 25-dihydroxyvitamin D-3; experimental autoimmune encephalomyelitis; multiple sclerosis; tolerogenic dendritic cells
Categories
Funding
- General Programme of the National Natural Science Foundation of China [81671177, 81471216]
- International Science and Technology Cooperation Programme of Jilin Provincial Science and Technology Development of China [20150414011GH]
- Innovation Programme of Jilin Provincial Health and Family Planning Commission of China [2016J040]
- Norman Bethune Cultivation Plan of Jilin University [2015320]
- Science and Technology Programme of Jilin Provincial Education Department of China [2016-462]
- Swedish Research Council [K2013-66X-22337-01-3, 2015-03005]
- First Hospital, Jilin University of China
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Dendritic cells (DCs), a bridge for innate and adaptive immune responses, play a key role in the development of multiple sclerosis (MS) and experimental autoimmune encephalomyelitis (EAE), an animal model for MS. Administration of tolerogenic DCs has been used as an immunotherapy in autoimmune diseases. Deficiency of vitamin D is an environmental risk factor of MS. In this study, we induced tolerogenic DCs by 1,25-dihydroxyvitamin D-3 and transferred the tolerogenic DCs (VD3-DCs) into EAE mice by adoptive transfer. We found that VD3-DCs inhibited the infiltrations of T helper type 1 (Th1) and Th17 cells into spinal cord and increased the proportions of regulatory T cells (CD4(+) CD25(+) Foxp3(+)), CD4(+) IL-10(+) T cells and regulatory B cells (CD19(+) CD5(+) CD1d(+)) in peripheral immune organs, which resulted in attenuated EAE. However, the proportions of T helper type 1 (Th1) and Th17 cells in spleen and lymph nodes and the levels of pro-inflammatory cytokines and IgG in serum also increased after transfer of VD3-DCs. We conclude that transfer of VD3-DCs suppressed EAE by increasing proportions of regulatory T cells, CD4(+) IL-10(+) T cells and regulatory B cells in spleen and reducing infiltration of Th1 and Th17 cells into spinal cord, which suggests a possible immunotherapy method using VD3-DCs in MS.
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