Journal
IMMUNOLOGICAL REVIEWS
Volume 275, Issue 1, Pages 11-20Publisher
WILEY
DOI: 10.1111/imr.12484
Keywords
B cells; HIV; monoclonal antibody isolation; neutralization
Categories
Funding
- Center for HIV/AIDS Vaccine Immunology and Immunogen Discovery Grant [UM1AI100663]
- Collaboration for AIDS Vaccine Discovery [OPP1084519, OPP1115782]
- European Union Marie-Curie Fellowship [FP7-PEOPLE-2013-IOF]
- IAVI
- USAID
- Bill AMP
- Melinda Gates Foundation
- NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES [UM1AI100663] Funding Source: NIH RePORTER
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Beginning in 2009, studies of the humoral responses of HIV-positive individuals have led to the identification of scores, if not hundreds, of antibodies that are both broadly reactive and potently neutralizing. This development has provided renewed impetus toward an HIV vaccine and led directly to the development of novel immunogens. Advances in identification of donors with the most potent and broad anti-HIV serum neutralizing responses were crucial in this effort. Equally, development of methods for the rapid generation of human antibodies from these donors was pivotal. Primarily these methods comprise single B-cell culture coupled to high-throughput neutralization screening and flow cytometry-based sorting of single B cells using HIV envelope protein baits. In this review, the advantages and disadvantages of these methodologies are discussed in the context of the specificities targeted by individual antibodies and the need for further improvements to evaluate HIV vaccine candidates.
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