Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 162, Issue -, Pages 599-608Publisher
ELSEVIER
DOI: 10.1016/j.ijbiomac.2020.06.146
Keywords
Sulfated polysaccharide; Periodate-chlorite oxidation; Polysaccharide conformation
Funding
- Fundacao Araucaria/CNPq (PRONEX-Carboidratos)
- Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES, Brazil) [001]
- National Council for Scientific and Technological Development (CNPq, Brazil)
- Fundacao Araucaria
- CAPES
Ask authors/readers for more resources
This study investigates conformational aspects of ulvans (F2) and their polycarboxyl derivatives obtained through periodate-chlorite oxidation (C3) followed by DEAE-Sephacel fractioning (C3b and C3c). Size exclusion chromatography coupled with laser light scattering and viscometric detection, in addition to circular dichroism (CD) and molecular modeling analyses, suggested that F2 had a compact sphere conformation with a helical motif as secondary structure. In contrast, all the polycarboxyl ulvans showed a random coil conformation, al-though C3c (NaSO3- 21.0%; COO- 1.81 mmol.g(-1); M-w 18 kg.mol(-1)) had a more rigid and constrained backbone than C3 (NaSO3- 21.0%; COO- 1.81 mmol.g(-1); M-w 49 kg.mol(-1)), largely due to its higher sulfate and carboxyl content. Despite the higher ionic character of C3c, its anticoagulant activity (ACA), determined by activated partial thromboplastin time (APTT) assay, was not improved compared to that of C3. Moreover, C3b (NaSO3- 14.1%; COO- 1.23 mmol.g(-1); M-w 8.1 kg.mol(-1)) had higher activity than F2 (NaSO3- 20.6.%; COO- 0.36 mmol.g(-1); M-w 123 kg.mol(-1)), even with its lower sulfate content and molar mass. These results suggest that the ACA of polycarboxyl ulvans relies on carboxyl and sulfate content and may depend, in addition, on a proper flexible conformation. (c) 2020 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available