Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 162, Issue -, Pages 394-404Publisher
ELSEVIER
DOI: 10.1016/j.ijbiomac.2020.06.144
Keywords
Proteases; Proteolysis; Synthesis of peptides; Bioactive peptides; Protease immobilization; Ficin
Funding
- Ministerio de Ciencia e Innovacion from Spanish Government [CTQ201786170-R]
- FPU fellowship (Ministerio de Educacion)
- Algerian Ministry of Higher Education and Scientific Research
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Due to the problems raised by the use of animal or microbial recombinant proteases, the use of proteases from vegetable origin is becoming increasingly popular.. Among them, sulfidryl proteases have a special interest. Ficin is an outstanding example of this kind of proteases. This paper aims to be to make a comprehensive review of the recent uses of this enzyme, including for example protein hydrolysis, the production of bioactive peptides and antibodies fragments (researchers point that ficin results are more reproducible than using other proteases), meat tenderization, milk coagulations in cheese making or peptide synthesis. Efforts to get industrial immobilized biocatalysts of the enzyme will be also described. The review shows the huge potential and brilliant prospect that this enzyme can have in the near future. (c) 2020 Elsevier B.V. All rights reserved.
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