Journal
CELLS
Volume 9, Issue 11, Pages -Publisher
MDPI
DOI: 10.3390/cells9112388
Keywords
lamin A; C; protein kinase C alpha; striated muscle laminopathies; DCM; EDMD; L-CMD
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Funding
- Heart and Stroke Foundation Grant [NA 6628]
- CIHR [DEV312217]
- INSERM
- Association Institut de Myologie (AIM)
- University of Ottawa's Faculty of Science graduate office
- University of Ottawa Centre for Research Opportunities office
- Sorbonne Universite-Medecine grants
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Striated muscle laminopathies are cardiac and skeletal muscle conditions caused by mutations in the lamin A/C gene (LMNA). LMNA codes for the A-type lamins, which are nuclear intermediate filaments that maintain the nuclear structure and nuclear processes such as gene expression. Protein kinase C alpha (PKC-alpha) interacts with lamin A/C and with several lamin A/C partners involved in striated muscle laminopathies. To determine PKC-alpha's involvement in muscular laminopathies, PKC-alpha's localization, activation, and interactions with the A-type lamins were examined in various cell types expressing pathogenic lamin A/C mutations. The results showed aberrant nuclear PKC-alpha cellular distribution in mutant cells compared to WT. PKC-alpha activation (phos-PKC-alpha) was decreased or unchanged in the studied cells expressing LMNA mutations, and the activation of its downstream targets, ERK 1/2, paralleled PKC-alpha activation alteration. Furthermore, the phos-PKC-alpha-lamin A/C proximity was altered. Overall, the data showed that PKC-alpha localization, activation, and proximity with lamin A/C were affected by certain pathogenic LMNA mutations, suggesting PKC-alpha involvement in striated muscle laminopathies.
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