Journal
FOOD CHEMISTRY
Volume 329, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2020.127224
Keywords
Label-free; Multi-readout; Nanozyme; Lateral flow immunoassay; Mannose modified Prussian blue; Escherichia coli O157:H7
Funding
- National Natural Science Foundation of China [21675127]
- Natural Science Foundation of Qinghai Province [2019-ZJ-904]
- Development Project of Qinghai Key Laboratory [2017-ZJ-Y10]
- Capacity Building Project of Engineering Research Center of Qinghai Province [2017-GX-G03]
- Shaanxi Provincial Science Fund for Distinguished Young Scholars [2018JC-011]
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To overcome the drawbacks of antibody labeling dependence and single-readout system in the conventional lateral flow immunoassays (LFIAs) as well as the non-targeted combination of new capture agents reported recently for pathogen detection, in this work, a multi-readout and label-free LFIA was proposed for rapid detection of Escherichia coli O157:H7 (E. coli O157:H7) based on a nanozyme-bacteria-antibody sandwich pattern. A type of functional nanozyme-mannose modified Prussian blue (man-PB), was introduced as the recognition agent as well as signal indicator. Apart from original signal intensity on the T-line, the peroxidase-like catalytic activity-driven generation of colorimetric signal could be used as another format of quantitation. Importantly, such LFIA could exhibit excellent performance for target pathogens detection separately with a quantitative range of 10(2)-10(8) cfu.mL(-1) and a low detection limit of 10(2) cfu.mL(-1) based on different readout formats, indicating the application potential of the proposed LFIA in real samples.
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