Journal
CURRENT ANALYTICAL CHEMISTRY
Volume 12, Issue 1, Pages 5-21Publisher
BENTHAM SCIENCE PUBL LTD
DOI: 10.2174/1573411011666150611184215
Keywords
Biosensors; enzyme; electrochemistry; immobilization techniques; modified electrodes
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Funding
- National High Technology Research and Development Program (863 Program) of China [2015AA020502]
- National Natural Science Foundation of China [21375066]
- Open Research Fund of State Key Laboratory of Bioelectronics, Southeast University
- Open Research Fund of Key Laboratory of Energy Thermal Conversion and Control of Ministry of Education, Southeast University
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This review introduces the concept of enzyme biosensors. As the major feature of an enzyme is its selectivity and specificity, we briefly highlighted the principles of enzyme production and enzyme classification. Moreover, enzyme immobilization methods, including physical adsorption and entrapment techniques with special attention on cross-linking and covalent binding are discussed followed by different electrochemical methods used in the biosensors fabrication. Furthermore, a comprehensive discussion about horseradish peroxidase (HRP) as a model enzyme is presented with investigation of some modified enzyme electrodes such as screen-printed and carbon paste electrodes. Some approaches to enhance biosensors performances and their applications are also highlighted.
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