Journal
PROCESS BIOCHEMISTRY
Volume 98, Issue -, Pages 21-33Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.procbio.2020.07.008
Keywords
Platinum nanoparticles; Tragia involucrata; Antioxidant; HeLa cells; Mitochondrial membrane potential
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Funding
- RUSA scheme Phase 2.0 grant, Dept of Education, Govt. of India. [F-24-51/2014-U]
- Basic Science Research Program through the National Research Foundation of Korea (NRF) - Ministry of Education [2018R1A6A1A03023584]
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This study aims to synthesis pharmaceutical potential platinum nanoparticles from Tragia involucrata leaf extract (Ti-PtNPs) for biomedical applications. The colour development in the reaction mixture confirms the integration of Ti-PtNPs and its surface plasmon resonance (SPR) peak observed at 252 nm. The crystalline nature of Ti-PtNPs was authenticated by X-ray diffraction (XRD) analysis. The functional groups present in the T. involucrata (AE-Ti) aqueous extract, notably polyphenols, alkaloids, flavonoids, and proteins, participated in the synthesis of Ti-PtNP5, established in the Fourier-transform infrared spectroscopy (FT-IR) analysis. The synthesized Ti-PtNP5 demonstrate spherical like particles with an average size of 10 nm. Ti-PtNP5 exhibited dose depended on antioxidant activities and the activities were higher than the AE-Ti. The antibacterial activity of Ti-PtNP5 was tested against Gram-positive (Staphylococcus aureus) and Gram-negative bacteria (Escherichia coli) and displays admirable inhibitory activity in agar bioassay, disc diffusion, killing kinetics. Also, the synthesized Ti-PtNP5 have attached the outer cell wall of bacteria and increased the protein leakage activity. The Ti-PtNP5 exhibited potent cytotoxicity against HeLa cells with an IC50 value of 27.72 mu g/ml. Moreover, the Ti-PtNP5 enhance the intracellular ROS production, which induces the mitochondria-mediated apoptosis. Thus, the synthesis Ti-PtNP5 can be used as a broad spectrum of pharmaceutical applications.
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