Journal
COLLOIDS AND SURFACES B-BIOINTERFACES
Volume 195, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.colsurfb.2020.111257
Keywords
Lubricin; Mucins; Dry eye; Tear film; Fluid film stability; Wettability; Marangoni flow; QCM-D; Adsorption
Funding
- Novartis Pharma AG
- National Science Foundation [ECCS-1542152]
- U.S. Department of Energy, Office of Science, Office of Basic Energy Sciences [DE-AC02-76SF00515]
- DOE Office of Biological and Environmental Research
- National Institutes of Health, National Institute of General Medical Sciences [P41GM103393]
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The wetting and adsorption properties for two glycoproteins, recombinant human lubricin and bovine submaxillary mucins (BSM) were evaluated on hydrophilic and hydrophobic glass dome surfaces in a simplified in vitro tear film model. We show that both recombinant human lubricin (rh-lubricin) and BSM solutions render surfaces hydrophilic and when the fluid films reach 500 nm or less, the fluids resist evaporation-driven breakup through a volumetric flux across the surface, which we believe is due to evaporation-driven solutocapillary flows. rh-Lubricin was able to maintain a wet film without spontaneous breakup for longer periods of time than BSM at lower concentrations, which we attribute to differences in adsorption properties, measured by QCM-D, that result from surface charge and structural differences (confirmed by zeta potential, DLS, and SAXS measurements).
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