Chemically robust succinimide-group-assisted irreversible bonding of poly(dimethylsiloxane)-thermoplastic microfluidic devices at room temperature

This study investigates surface chemical modification using anhydride silane and amino silane reagents at room temperature (RT) to realize bonding between silicon-based PDMS and non-silicon thermoplastics. The anhydride silane shows vigorous activity against water, forming a terminal dicarboxylic acid in the plasma-activated elastomeric poly(dimethylsiloxane) (PDMS) surface, and it can readily react with amino-silane-modified thermoplastic surfaces, resulting in a permanent bond via the formation of a stable succinimide group without the requirement for high temperature or additional pressure to initiate the bonding. The modified surfaces of PDMS and thermoplastics were successfully characterized by water contact angle measurement, fluorescence measurement, X-ray photoelectron spectroscopy (XPS), and atomic force microscopy (AFM). The bond strength values of PDMS-thermoplastic assemblies, measured by the tensile test for PDMS-polystyrene (PS), PDMS-poly(methyl methacrylate) (PMMA), PDMS-polycarbonate (PC), and PDMS-poly(ethyl terephthalate) (PET) assemblies, were found to be approximately 519.5 +/- 6, 259 +/- 15, 476.6 +/- 8, and 458.2 +/- 27 kPa, respectively. Moreover, the bond strength was further examined by performing a burst test for PDMS-PMMA, PDMS-PS, PDMS-PC, and PDMS-PET microfluidic devices, which were found to have the maximum pressure values at approximately 344.73, 448.15, 413.68, and 379.21 kPa, respectively. Based on these results, the hybrid microfluidic devices can be used for high-pressure experiments such as blood plasma separation and continuous-flow polymerase chain reaction (CF-PCR). We have also performed the large area bonding of the PDMS-PC assembly (10 x 10 cm(2)), ensuring the high robustness and reliability of the proposed surface chemical bonding method.