Journal
SCIENCE OF THE TOTAL ENVIRONMENT
Volume 743, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.scitotenv.2020.140621
Keywords
Wastewater-based epidemiology; SARS-CoV-2; Surveillance; COVTD-19; RT-qPCR; Wastewater
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Funding
- Board of Regents grants [LEQSF (2018-21)-rd-a-21, LEQSF (2019-23)-ENH-DE-15]
- Japan Society for the Promotion of Science (JSPS) through the Promotion of Joint International Research (Fostering Joint International Research (B)) [JP18KK0270]
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We investigated the presence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA in wastewater samples in southern Louisiana, USA. Untreated and treated wastewater samples were collected on five occasions over a four-month period from January to April 2020. The wastewater samples were concentrated via ultrafiltration (Method A). and an adsorption-elution method using electronegative membranes (Method B). SARS-CoV-2 RNA was detected in 2 out of 15 wastewater samples using two reverse transcription-quantitative polymerase chain reaction (RT-qPCR) assays (CDC N1 and N2). None of the secondary treated and final effluent samples tested positive for SARS-CoV-2 RNA. To our knowledge, this is the first study reporting the detection of SAR.S-CoV-2 RNA in wastewater in North America, including the USA. However, concentration methods and RTqPCR assays need to be refined and validated to increase the sensitivity of SARS-CoV-2 RNA detection in wastewater. (C) 2020 Elsevier B.V. All rights reserved.
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