Journal
CHROMOSOMA
Volume 129, Issue 3-4, Pages 275-283Publisher
SPRINGER
DOI: 10.1007/s00412-020-00744-7
Keywords
Fluorescence in situ hybridization; Robertsonian translocation; Parthenogenesis; Reticulate evolution; Whole-chromosome painting; Comparative genomic hybridization; Pericentromeric DNA; Interstitial telomeric sites
Funding
- RFBR [17-00-00430, 17-00-00429, 18-54-05020]
- SCS MES RA-RFFR [18RF-132]
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) [401962/2016-4, 302449/2018-3]
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [2018/22033-1]
- CAPES/Alexander von Humboldt [88881.136128/2017-01]
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Darevskia rostombekowi, the most outstanding of the seven known parthenogenetic species in the genus Darevskia, is the result of an ancestral cross between two bisexual species Darevskia raddei and Darevskia portschinskii. The chromosomal set of this species includes a unique submetacentric autosomal chromosome; the origin of this chromosome was unresolved as only acrocentric chromosomes are described in the karyotypes of Darevskia genus normally. Here, we applied a suite of molecular cytogenetic techniques, including the mapping of telomeric (TTAGGG) n repeats using fluorescence in situ hybridization (FISH), comparative genomic hybridization (CGH), and whole-chromosome painting (WCP) in both D. rostombekowi and parental (D. portschinskii and D. raddei) species. The obtained results in total suggest that a de novo chromosomal rearrangement via Robertsonian translocation (centric fusion) between two maternal (D. raddei) acrocentric chromosomes of different size was involved in the formation of this unique submetacentric chromosome present in the parthenogenetic species D. rostombekowi. Our findings provide new data in specific and rapid evolutional processes of a unisexual reptile species karyotype.
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