Journal
EXPERIMENTAL CELL RESEARCH
Volume 396, Issue 1, Pages -Publisher
ELSEVIER INC
DOI: 10.1016/j.yexcr.2020.112298
Keywords
Telomerase reverse transcriptase; TERTp mutations; TERT expression; Telomerase activity; Telomere length; Neoplastic haematological and solid tumour human cell lines
Categories
Funding
- National Centre for Research and Development, Warsaw, Poland [STRATEGMED3/306853]
Ask authors/readers for more resources
Promoter region of the telomerase reverse transcriptase gene (TERTp) constitutes a regulatory element capable to affect TERT expression (TE), telomerase activity (TA) and telomere length (TL). TERTp mutation status, TL, TA and TE were assessed in 27 in vitro cultured human cell lines, including 11 solid tumour, 13 haemato-logical and 3 normal cell lines. C228T and C250T TERTp mutations were detected in 5 solid tumour and none of haematological cell lines (p = 0.0100). As compared to other solid tumour cell lines, those with the presence of somatic mutations were characterized by: shorter TL, lower TA and TE. Furthermore, cell lines carrying TERTp mutations showed a linear correlation between TE and TA (R = 0.9708, p = 0.0021). Moreover, haematological cell lines exhibited higher TE compared to solid tumour cell lines (p = 0.0007). TL and TA were correlated in both solid tumour (R = 0.4875, p = 0.0169) and haematological (R = 0.4719, p = 0.0095) cell lines. Our results based on the in vitro model suggest that oncogenic processes may differ between solid tumours and haematological malignancies with regard to their TERT gene regulation mechanisms.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available