Journal
CELL DEATH & DISEASE
Volume 11, Issue 10, Pages -Publisher
SPRINGERNATURE
DOI: 10.1038/s41419-020-03152-y
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Funding
- Ministry of Science and Technology of the People's Republic of China [2018YFA0800700]
- National Natural Science Foundation of China [91854107, 31671393, 31970711]
- Fundamental Research Funds for the Central Universities
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Mitochondrial cristae are the main site for oxidative phosphorylation, which is critical for cellular energy production. Upon different physiological or pathological stresses, mitochondrial cristae undergo remodeling to reprogram mitochondrial function. However, how mitochondrial cristae are formed, maintained, and remolded is still largely unknown due to the technical challenges of tracking mitochondrial crista dynamics in living cells. Here, using live-cell Hessian structured illumination microscopy combined with transmission electron microscopy, focused ion beam/scanning electron microscopy, and three-dimensional tomographic reconstruction, we show, in living cells, that mitochondrial cristae are highly dynamic and undergo morphological changes, including elongation, shortening, fusion, division, and detachment from the mitochondrial inner boundary membrane (IBM). In addition, we find that OPA1, Yme1L, MICOS, and Sam50, along with the newly identified crista regulator ATAD3A, control mitochondrial crista dynamics. Furthermore, we discover two new types of mitochondrial crista in dysfunctional mitochondria, cut-through crista and spherical crista, which are formed due to incomplete mitochondrial fusion and dysfunction of the MICOS complex. Interestingly, cut-through crista can convert to lamellar crista. Overall, we provide a direct link between mitochondrial crista formation and mitochondrial crista dynamics.
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