Journal
MOLECULES
Volume 25, Issue 21, Pages -Publisher
MDPI
DOI: 10.3390/molecules25215037
Keywords
Zika virus; dengue virus; andrographolide; HSPA1A; PGK1
Funding
- Newton Fund [FDA-CO-2561-6820-TH]
- Thailand Research Fund [BRG6080006]
- National Research Council of Thailand
- Mahidol University [NRCT5-RSA63015-03]
- Thailand Graduate Institute of Science and Technology (TGIST)
Ask authors/readers for more resources
Andrographolide is a labdene diterpenoid with potential applications against a number of viruses, including the mosquito-transmitted dengue virus (DENV). In this study, we evaluated the anti-viral activity of three 14-aryloxy analogues (ZAD-1 to ZAD-3) of andrographolide against Zika virus (ZIKV) and DENV. Interestingly, one analogue, ZAD-1, showed better activity against both ZIKV and DENV than the parental andrographolide. A two-dimension (2D) proteomic analysis of human A549 cells treated with ZAD-1 compared to cells treated with andrographolide identified four differentially expressed proteins (heat shock 70 kDa protein 1 (HSPA1A), phosphoglycerate kinase 1 (PGK1), transketolase (TKT) and GTP-binding nuclear protein Ran (Ran)). Western blot analysis confirmed that ZAD-1 treatment downregulated expression of HSPA1A and upregulated expression of PGK1 as compared to andrographolide treatment. These results suggest that 14-aryloxy analogues of andrographolide have the potential for further development as anti-DENV and anti-ZIKV agents.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available