Journal
JOURNAL OF HELMINTHOLOGY
Volume 94, Issue -, Pages -Publisher
CAMBRIDGE UNIV PRESS
DOI: 10.1017/S0022149X20000930
Keywords
Strongyloides; nematode; egg; purification; method; faeces
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Funding
- Edital MCT/CNPq-Brazil [142056/2018-9]
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo, Brazil [2016/06185-0]
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The aim of this study was to develop a simple method to purify Strongyloides eggs from rat faeces using a sucrose gradient centrifugal-flotation technique. This procedure is simple, rapid and possesses a high efficiency in recovering Strongyloides eggs without faecal detritus in less than one hour, thus eliminating the use of complex apparatus and different chemical substances. The possibility of working with pure and live Strongyloides eggs opens up a wide range of future studies on the biology of this parasite. This study constitutes the first report in the scientific literature on purifying Strongyloides eggs using a sucrose density gradient.
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