Journal
BRAZILIAN JOURNAL OF ANALYTICAL CHEMISTRY
Volume 7, Issue 29, Pages 64-83Publisher
VISAO FOKKA COMMUNICATION AGENCY
DOI: 10.30744/brjac.2179-3425.AR-41-2020
Keywords
MALDI-TOF MS; MALDI Imaging; Tumor biomarkers; Glioblastoma multiforme; In situ proteomics
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Funding
- Sao Paulo State Research Foundation (FAPESP) [2016/16212-5]
- INCT Program/iii-CNPq/MCT [305566/2017-2]
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In order to evaluate the use of matrix-assisted laser desorption ionization (MALDI) Mass Spectral Imaging (MSI) to Glioblastoma (GBM) studies, some sections of formalin fixed paraffin embedded samples of GBM tumors were submitted to classical immunoassays, to define the profile of distribution of some classical and well recognized molecular markers of GBM grade IV (Ki-67, S100, Glial GFAP, CD31 and CD34), while other sections of the same samples were submitted to in-tissueproteomic analysis by MALDI MSI, and both results compared to each other. The overlapping of the MALDI spectra obtained for the tryptic peptides with the immunohistochemical reactions of each marker protein were used to build a distribution map of the marker proteins all over the GBM tissue section. The results revealed a high correlation between both methods, indicating that MALDI MSI has enough sensitivity to be compared to the immunohistochemical methods, as well is sufficiently reliable to be used in biomarkers identification.
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