Journal
ANTICANCER RESEARCH
Volume 40, Issue 11, Pages 6151-6158Publisher
INT INST ANTICANCER RESEARCH
DOI: 10.21873/anticanres.14635
Keywords
Acetylenic synthetic betulin derivatives; betulin; chemotherapy; glioma
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Funding
- Medical University in Lublin [DS440]
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Background/Aim: Glioma is the most malignant tumour of the human brain still lacking effective treatment modalities. Betulin, a pentacyclic triterpene abundantly found in the birch bark, has been shown to demonstrate interesting anti-cancer activity towards many cancer cells. We determined the effects of acetylenic synthetic betulin derivatives (ASBDs) as anti-tumour agents on glioma cells in vitro. Materials and Methods: T98G and C6 glioma cell viability and proliferation were determined by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assay and BrdU (bromo deoxyuridine) test, respectively. Cell-cycle progression and induction of apoptosis were investigated with flow cytometry. Results: ASBDs significantly decreased glioma cell viability/survival and inhibited proliferation in a dose-dependent manner in vitro. Moreover, ASBDs were more cytotoxic than clinically used chemotherapeutics - temozolomide and cisplatin. Conclusion: ASBDs may be considered for further study as potent anti-tumour agents in glioma treatment.
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