Journal
CELL DEATH & DISEASE
Volume 11, Issue 11, Pages -Publisher
SPRINGERNATURE
DOI: 10.1038/s41419-020-03178-2
Keywords
-
Categories
Ask authors/readers for more resources
Idiopathic pulmonary fibrosis (IPF) is featured with inflammation and extensive lung remodeling caused by overloaded deposition of extracellular matrix. Scutellarin is the major effective ingredient of breviscapine and its anti-inflammation efficacy has been reported before. Nevertheless, the impact of scutellarin on IPF and the downstream molecular mechanism remain unclear. In this study, scutellarin suppressed BLM-induced inflammation via NF-kappa B/NLRP3 pathway both in vivo and in vitro. BLM significantly elevated p-p65/p65 ratio, I kappa B alpha degradation, and levels of NLRP3, caspase-1, caspase-11, ASC, GSDMD(Nterm), IL-1 beta, and IL-18, while scutellarin reversed the above alterations except for that of caspase-11. Scutellarin inhibited BLM-induced epithelial-mesenchymal transition (EMT) process in vivo and in vitro. The expression levels of EMT-related markers, including fibronectin, vimentin, N-cadherin, matrix metalloproteinase 2 (MMP-2) and MMP-9, were increased in BLM group, and suppressed by scutellarin. The expression level of E-cadherin showed the opposite changes. However, overexpression of NLRP3 eliminated the anti-inflammation and anti-EMT functions of scutellarin in vitro. In conclusion, scutellarin suppressed inflammation and EMT in BLM-induced pulmonary fibrosis through NF-kappa B/NLRP3 signaling.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available