Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 163, Issue -, Pages 476-484Publisher
ELSEVIER
DOI: 10.1016/j.ijbiomac.2020.06.206
Keywords
Ganoderma spp. polysaccharides; Fingerprints; Multivariate analysis
Funding
- Key Project at Central Government Level: The ability establishment of sustainable use for valuable Chinese medicine resources [2060302]
- Young Elite Scientist Sponsorship Program by China Association for Science and Technology [CAST-2018-QNRC1-04, QNRC1-04]
- Key Project of Shandong Province [2018YYSP012, 2019GSF108062]
- National Natural Science Foundation of China [81603293]
- Fundamental Research Funds for the Central public welfare research institutes (China) [ZZ13-YQ-090]
- Taishan Scholar Project of Shandong Province
- Priority Research Program of the Shandong Academy of Sciences
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In this preliminary study, the acidic hydrolysate fingerprints of polysaccharides based on hydrophilic-interaction chromatography-evaporative light scattering detection-electrospray time-of-flight mass spectrometry (HILIC-ELSD/ESI-TOF/MS) combined with multivariate statistical analysis was developed and applied to investigate the quality of Ganoderma lucidum from different regions. Projection-to-latent-structure discrimination analysis (PLS-DA) could distinguish samples of Zhejiang regions from those of other regions. Orthogonal-projection-to-latent-structure discrimination analysis (OPLS-DA) provided clear discrimination between G. lucidum samples cultivated in Zhejiang and that from other regions, in which Polysaccharides and D-galactose could be considered as candidate biomarkers. In addition, the intraspecific differentiation of G. lucidum was preliminarily investigated with samples from Shaanxi region. They were classified into four groups by PCA and PLS-DA, in which L-rhamnose, D-xylose, L-arabinose, and mannose were considered as potential chemical markers. These preliminary results contributed to our understanding of the variance of polysaccharides in Ganoderma spp. from different geographic origins and the intraspecific differentiation from the same region, which suggest great potential in the quality control of Ganoderma spp. (C) 2020 Elsevier B.V. All rights reserved.
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