Journal
STAR PROTOCOLS
Volume 1, Issue 3, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.xpro.2020.100191
Keywords
-
Categories
Funding
- KAKENHI from Japan Society for the Promotion of Science (JSPS) [JP25221004, 15H05774, 20H00513, 19K16604, 20K16212]
- Innovative Area on Integrated Analysis and Regulation of Cellular Diversity [17H06326]
- Japan Society for the Promotion of Science (JSPS)
- Brain/MINDS [JP21dm0207049]
- Science and Technology Platform Program for Advanced Biological Medicine from Japan Agency for Medical Research and Development (AMED)
- HFSP Research Grant Program [JPMJER2001]
- Human Frontiers Human Frontier Science Program (HFSP)
- Takeda Science Foundation
- ERATO [JP21am0401011]
- Japan Science and Technology Agency (JST)
- [HFSP RGP0019/2018]
Ask authors/readers for more resources
Tissue-clearing technologies have developed rapidly in the past decade, espe-cially for use in neuroscience research. We previously reported that CUBIC, which is one tissue-clearing method, is useful for applications in cancer research. CUBIC cancer analysis can be used to detect cancer metastasis with single-cell resolution at whole mouse body/organ level. This approach can also analyze the tumor char-acteristics with high-quality 3D images. Here, we describe a detailed CUBIC can-cer protocol from tissue clearing, capturing 3D images and post-immunohisto-chemistry. For complete details on the use and execution of this protocol, please refer to Kubota et al. (2017).
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available