Journal
STAR PROTOCOLS
Volume 1, Issue 3, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.xpro.2020.100115
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Funding
- National Natural Science Foundation of China [81725018, 81930088, 81703118, 81803123]
- Shanghai Collaborative Innovation Center for Translational Medicine [TM201925]
- Innovative Research Team of High -Level Local Universities in Shanghai
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Psoriasis is an incurable chronic inflammatory skin disorder. The imiquimod (IMQ)-induced mouse model of psoriasis is the most widely used model for drug discovery and pre-clinical studies of psoriasis. The inflamed and thickened skin frequently compromises the quality of single-cell suspensions generated from IMQ-induced skin lesions, which has an impact on subsequent analyses by flow cytometry. This protocol details the complete procedure for the establish-ment of a mouse model of psoriasis and flow cytometric detection of immune cells in the inflamed epidermis and dermis. For complete details on the use and execution of this protocol, please refer to Lou et al. (2020).
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