Journal
STAR PROTOCOLS
Volume 1, Issue 3, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.xpro.2020.100226
Keywords
basic pH; mimicking; beneficial plant; plant growth; dependent plant-
Categories
Funding
- Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) under Germany's Excellence Strategy [2048/1, 390686111]
- Euro-pean Research Council advanced grant (ROOTMICROBIOTA) , a RIKEN grant
- European Research Council
- RIKEN grant
- Dong-A University - Republic of Korea
- Max Planck Society
- JSPS KAKENHI [20K05955, 15J04093]
- Japan Science and Technology Agency (JST) -PRESTO [JPMJPR16Q2]
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We present a gnotobiotic system for microbiota reconstitution on Arabidopsis thaliana under contrasting iron availability. This system induces iron starvation in plants by providing an unavailable form, mimicking conditions in alkaline soils. Inoculation of taxonomically diverse bacteria reconstitutes plants with a synthetic microbiota, allowing observation of nutrient-dependent interactions with commensals. Experimental optimization, including media composition and preparation of seedlings and bacteria, is discussed. This system provides a framework that can be adapted to study plant-microbiota interactions in further nutritional contexts.For complete details on the use and execution of this protocol, please refer to Harbort et al. (2020).
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