Journal
STAR PROTOCOLS
Volume 1, Issue 3, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.xpro.2020.100221
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Funding
- Institutional Development Award (IDeA) from the National Institute of General Medical Sciences of the National Institutes of Health [2P20GM103432]
- NIGMS [R01GM113028]
- NSF Faculty CAREER Program [BBBE 1254608]
- Whitman Center fellowships
- Marine Biological Laboratory
- Biomedical Scholars program
- Pew Charitable Trusts
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Cell-free extract derived from the eggs of the African clawed frog Xenopus laevis is a well-established model system that has been used historically in bulk aliquots. Here, we describe a microfluidic approach for isolating discrete, biologically relevant volumes of cell-free extract, with more expansive and precise control of extract shape compared with extract-oil emulsions. This approach is useful for investigating the mechanics of intracellular processes affected by cell geometry or cytoplasmic volume, including organelle scaling and positioning mechanisms.For complete details on the use and execution of this protocol, please refer to Geisterfer et al. (2020).
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