Journal
STAR PROTOCOLS
Volume 1, Issue 3, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.xpro.2020.100194
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Funding
- JSPS KAKENHI [17K1494117K14941]
- OIST Graduate University
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Chronic cranial window surgery is a critical procedure for in vivo imaging in neuroscience. Here, we describe our surgical protocol with several subtle im-provements that increase the success rate significantly. The window allows high-quality imaging in head-fixed behaving mice within the first week after the surgical procedure and remains clear for months. We used this procedure to prepare mice for intrinsic signal imaging and two-photon imaging of layer 6 neurons in visual cortex.For complete details on the use and execution of this protocol, please refer to Augustinaite and Kuhn (2020).
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