Journal
ANALYTICAL METHODS
Volume 12, Issue 46, Pages 5613-5620Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/d0ay01137a
Keywords
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Funding
- Key Laboratory of Regenerative Biology, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Stem Cell and Molecular Diagnostic Laboratory
- National Natural Science Foundation of China [31671933]
- Guizhou Medical University Graduate Student Innovation Program [[2019] 001]
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The pathogen of viral hemorrhagic fever (VHF), which is harmful to human health, is a hemorrhagic fever virus. Clinicians have long needed convenient and sensitive point-of-care rapid diagnostic tests (RDTs) for hemorrhagic fever viruses. Commonly used methods for pathogen detection rely on conventional culture-based tests, antibody-based assays and polymerase chain reaction (PCR)-based techniques. However, these methods are costly, laborious and time-consuming. Herein, we present a simple and sensitive biosensor for the rapid detection of hemorrhagic fever viruses. For this assay, we develop lateral flow biosensors (LFBs) based on magnetic beads and nicking enzyme-assisted isothermal strand-displacement amplification (SDA) for the detection of hemorrhagic fever viruses. The detection limit of this assay is 10 fM.
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