Journal
ORAL ONCOLOGY
Volume 110, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.oraloncology.2020.104943
Keywords
Tumor mutation burden (TMB); Bead and neck squamous cell carcinoma (HNSCC); TCGA; Tumor microenvironment; Prognosis
Categories
Funding
- National Natural Science Foundation of China [81472521, 81402251, 81502350, 81672676, 81772890]
- Guangdong Science and Technology Development Fund [2015A030313181, 2016A030313352, 2017A030311011]
- Science and Technology Program of Guangzhou [201607010108, 201803010060]
- Fundamental Research Funds for the Central Universities [16ykpy10, 19ykzd20]
- National Clinical Key Specialty Construction Project for Department of Oral and Maxillofacial Surgery
- Key Laboratory of Malignant Tumor Gene Regulation and Target Therapy of Guangdong Higher Education Institutes, Sun-Yat-Sen University [KLB09001]
- Key Laboratory of Malignant Tumor Molecular Mechanism and Translational Medicine of Guangzhou Bureau of Science and Information Technology [(2013)163]
- Sun Yat-sen University Key Teacher Cultivation Project [19ykzd22]
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Objective: Whether tumor mutation burden (TMB) affects prognosis and immune infiltration of tumor patients is controversial. We designed and conducted a multi-omics study with the aim of investigating the prognostic value of TMB and the relationship between TMB and immune infiltration in head and neck squamous cell carcinoma (HNSCC). Methods: TMB scores were calculated from the mutation data of 506 HNSCC samples from The Cancer Genome Atlas (TCGA), and the patients were divided into low- and high-TMB groups according to the TMB score quartiles. Differentially expressed genes (DEGs) between the low-TMB and high-TMB groups were identified. Immune cell infiltration and survival analyses were conducted between groups. Results: High TMB in HNSCC patients was associated with a poor prognosis, large primary tumor size, advanced clinical stage and a human papillomavirus (HPV)-negative status. A total of 576 DEGs were identified, and gene set enrichment analysis (GSEA) revealed that the DEGs in the low-TMB group were enriched in immune-related pathways. Four hub genes were significantly associated with prognosis, and mutations in these genes affected immune infiltration. The estimated fractions of B memory cells and CD4 + memory resting cells were higher in the low-TMB group than in the high-TMB group, and B cell and CD4 + T cell infiltration was positively correlated with prognosis in HNSCC patients. Conclusions: HNSCC patients with low TMB have better prognoses than those with high TMB, and TMB might affect B cell and CD4 + T cell infiltration.
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