Journal
G3-GENES GENOMES GENETICS
Volume 10, Issue 12, Pages 4707-4712Publisher
GENETICS SOCIETY AMERICA
DOI: 10.1534/g3.120.401800
Keywords
cell competition; cell death; Eiger/TNF; NimC1 mutations; phagocytosis
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Funding
- Bloomington Drosophila Stock Center (BDSC) [NIH P40OD018537]
- NCI [R01CA192838]
- NIH [R01GM078464]
- NSF
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The process of apoptosis in epithelia involves activation of caspases, delamination of cells, and degradation of cellular components. Corpses and cellular debris are then rapidly cleared from the tissue by phagocytic blood cells. In studies of the Drosophila TNF, Eiger (Egr) and cell death in wing imaginal discs, the epithelial primordia of fly wings, we noticed that dying cells appeared to transiently accumulate in egr(3) mutant wing discs, raising the possibility that their phagocytic engulfment by hemocytes was impaired. Further investigation revealed that lymph glands and circulating hemocytes from egr(3) mutant larvae were completely devoid of NimC1 staining, a marker of phagocytic hemocytes. Genome sequencing uncovered mutations in the coding region that are predicted to truncate the NimC1 protein before its transmembrane domain, and provide an explanation for the lack of NimC staining. The work that we report here demonstrates the presence of these mutations in the widely used egr(3) mutant, its sister allele, egr(1), and its parental strain, Regg1(GS9830). As the egr(3) and egr(1) alleles have been used in numerous studies of immunity and cell death, it may be advisable to re-evaluate their associated phenotypes.
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