Journal
ANALYTICA CHIMICA ACTA
Volume 1138, Issue -, Pages 123-131Publisher
ELSEVIER
DOI: 10.1016/j.aca.2020.09.025
Keywords
Tetrahedral DNA nanostructure; Fe3O4 nanoparticles; Thionine; Graphene oxide; Electrochemical aptasensor
Categories
Funding
- National Natural Science Foundation of China [61301037]
- Program for Science and Technology Innovation Talents in Universities of Henan Province [20HASTIT002]
- Cultivation Plan for Young Core Teachers in Universities of Henan Province [2017GGJS072]
- National Engineering Laboratory for Wheat & Corn Further Processing, Henan University of Technology [NL2018004]
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In this work, we proposed an electrochemical aptasensor for patulin (PAT) based on tetrahedral DNA nanostructures (TDNs) and thionine (Thi)-labeled Fe3O4 nanoparticles (Fe(3)O(4)NPs)/rGO signal amplification strategy. The rigid structure of TDNs could effectively improve the binding efficiency. Fe(3)O(4)NPs/rGO with excellent electrical conductivity and large specific surface area was used as a label material, which could load more Thi and accelerate electron transfer. Besides, the unique catalytic properties of Fe(3)O(4)NPs could achieve active signal amplification. Once PAT existed, PAT aptamer was released from the capture probe, thereby introducing Fe(3)O(4)NPs/rGO with Thi onto the electrode surface. Therefore, a noticeable increase in Thi current intensity was observed. Under the optimized conditions, the proposed aptasensor showed superior performance with a linear range from 5 x 10(-8) to 5 x 10(-1) mg mL(-1) and a detection limit of 30.4 mu g mL(-1). The obtained sensor showed reliable specificity, stability and reproducibility, and was successfully applied to the determination of real samples. (C) 2020 Elsevier B.V. All rights reserved.
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