Journal
ANALYTICA CHIMICA ACTA
Volume 1137, Issue -, Pages 143-155Publisher
ELSEVIER
DOI: 10.1016/j.aca.2020.09.050
Keywords
Phytohormones; Ionic liquid; Carbon fibers; Solid-phase microextraction; Liquid chromatography-mass spectrometry
Categories
Funding
- National Natural Science Foundation of China [21775134, 51703192]
- 111 Project [D18012]
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Phytohormones are interrelated by synergistic or antagonistic crosstalk and play important roles in the regulation of plant growth and development. In order to understand the interaction between phytohormones in the plant physiological network, it is necessary to determine trace levels of multiple phytohormones simultaneously in a complex matrix. Here, we synthesized ionic liquids containing different functional groups and modified the surface of carbon fibers with them. Based on these carbon fibers-ionic liquid (CFs-IL) materials, a solid phase microextraction method was developed to enable the simultaneous extraction of phytohormones. The adsorption specificity of multiple phytohormones was studied by identifying the hydrophobic, electrostatic, and pi-pi interactions, as well as hydrogen bonds, which favor simultaneous extraction of the relevant acidic, alkaline and neutral phytohormones by improving compatibility. The proposed method, coupled with liquid chromatography-tandem mass spectrometry, was applied to the simultaneous determination of 13 acidic, alkaline and neutral phytohormones in tomato. The limits of quantification were found to be in the range of 0.32-54.05 ng mL(-1) and 4.6-185.8 pg mL(-1), respectively, when measured by QQQ and Q-TOF. All of the relative recoveries were in the range of 94.40-113.37% with RSDs <15.36% (n = 3) for spiked tomato samples. This method is expected to be widely applied to multiple phytohormones analysis for in-depth researches concerning the physiological networks of plants. (C) 2020 Elsevier B.V. All rights reserved.
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