Yellow mombin pulp residue valorization for pectinases production by Aspergillus niger IOC 4003 and its application in juice clarification

Pectinases are enzymes responsible for cleaving pectin and other pectic polysaccharides in galacturonic acid monomers. They are widely applied in industrial sectors. In this context, the present study evaluated polygalacturonase (PG), pectin lyase (PMGL) and FPase production by Aspergillus niger IOC 4003 in solid-state fermentation using yellow mombin pulp residue as substrate. Three parameters were investigated for extraction of PG such as temperature, pH stability and ionic strength. The action of PG was assessed under different states of temperature and pH as well in yellow mombin juice clarification. The antibacterial activity of yellow mombin juice was additionally evaluated. Results revealed that the highest yield of PG produced (38.22 +/- 0.63 U/g) was achieved at 72 h fermentation using washed yellow mombin pulp residue. Characterization studies indicated stability of PG for 2 h at 30 and 40 degrees C with pHs between 4 and 6. The PG applied in the yellow mombin was able to reduce turbidity by 41% and viscosity by 52%. Also, it was able to increase color by 36% and antioxidant capacity by 39%. Finally, the yellow mombin juice exhibited antibacterial activity against Staphylococcus aureus, Enterococcus faecalis, Escherichia coli and Klebsiella pneumoniae. Hence, this study demonstrated the feasibility of using tropical fruit residues from industries for pectinases synthesis and showed their application in the food industry.