4.6 Article

Lignin Degradation and Its Use in Signaling Development by the Coprophilous Ascomycete Podospora anserina

Journal

JOURNAL OF FUNGI
Volume 6, Issue 4, Pages -

Publisher

MDPI
DOI: 10.3390/jof6040278

Keywords

biomass; fungal degradation mechanisms; Podospora anserina; peroxide; pyrolysis-GCMS; miscanthus

Funding

  1. Universite Paris 7
  2. Universite Paris 13
  3. Programmes InterDisclipinaires of COMUE Sorbonne Paris Cite, grant Olizero from project Energies, territoire, societe: enjeux et approches croisees
  4. Region Ile de France, grant P3AMB
  5. DIM Reseau de Recherche sur le Developpement Soutenable-CNRS

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The filamentous fungus Podospora anserina is a good model to study the breakdown of lignocellulose, owing to its ease of culture and genetical analysis. Here, we show that the fungus is able to use a wide range of lignocellulosic materials as food sources. Using color assays, spectroscopy and pyrolysis-gas chromatography mass spectrometry, we confirm that this ascomycete is able to degrade lignin, primarily by hydrolyzing beta-O-4 linkages, which facilitates its nutrient uptake. We show that the limited weight loss that is promoted when attacking Miscanthus giganteus is due to a developmental blockage rather than an inefficiency of its enzymes. Finally, we show that lignin, and, more generally, phenolics, including degradation products of lignin, greatly stimulate the growth and fertility of the fungus in liquid cultures. Analyses of the CAT(Delta Delta Delta Delta Delta) mutant lacking all its catalases, pro-oxidants and antioxidants indicate that improved growth and fertility of the fungus is likely caused by augmented reactive oxygen species levels triggered by the presence of phenolics.

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